Microbial ID - Pricing

Pricing

DNA Sequencing of Bacteria, Yeast and Fungi
Number of samples	Price per sample
1-9	$108
10+	Call for a price quote
Full Gene Sequencing*	Call for a price quote
GenBank Search (as needed)	Included
PCR Optimization PCR Optimization: In our experience approximately ≤ 10% of samples fail PCR amplification on the 1st attempt. The most common cause of PCR failure is insufficient DNA concentration. By optimizing the concentration we can in most cases successfully amplify the DNA. If we are unable to amplify the DNA, there will be a total charge of $25 per sample.	$25
Comparison Report	$40 (per report)
Priority Services (DNA Sequencing)
Surcharge	Next Day Priority turnaround - $100/sample Same Day Priority turnaround - call for pricing 3 Day Priority turnaround - $30/sample Weekend Priority turnaround - call for details Other Priority services available- call for details and pricing
Fatty Acid Analysis (FAME) of Bacteria, Yeast and Non-Microbial Samples
Number of samples	Price per sample
1-9	$75
10+	Call for a price quote
Direct Fatty Acid Analysis Direct Fatty Acid Analysis: GC-FAME analysis of any suitable organic substance. Includes peak names and percentages. No additional sub-culturing required. Priority turnaround services not applicable.	Call for details and a price quote
DendrogramDendrogram: graphic comparison of samples to determine relatedness; strain-tracking	$50 (per dendrogram)
DendrogramDendrogram: graphic comparison of samples to determine relatedness; strain-tracking w/ previous data	$70 (per dendrogram)
Priority Services (FAME)
Surcharge	3 Day Priority turnaround - $25/sample
Polyphasic Analysis (FAME & DNA Sequencing)
Number of samples	Price per sample
1-9	$173
10+	Call for a price quote
Additional services and Fees
Gram Stain/Biochem./Coagulase Gram stain/Biochem/Coagulase: required confirmatory tests for E. coli, Salmonella and S. aureus FAME matches. Gram stains also available by request.	$20/sample MorphologyMorphology: Morphology of an organism will be evaluated where there is good colony separation (usually the 4th quadrant of a quadrant streak). Morphology is divided into 6 categories: Color - the overall color of the colony Shape - the overall shape of the colony Margin - the edge of the colony Density - whether or not you can see through the colony (media independent) Elevation - refers to the way the colony rises above the media Size Measure the size of one single colony	$30/sample
Confirmation MicroscopyConfirmation Microscopy: identification by traditional microscopy. Expect 1-2 week TAT. Requires DNA Sequencing be performed first. of Fungi Species	$85/sample TPC w/ serial dilutionsTPC w/serial dilutions: for very contaminated or highly concentrated samples, material is diluted to obtain accurate microbial counts.	$125/sample
Purification and Isolation (as needed)	$15/sample Processing Fee	$30/batch
MIDI Labs offers discounts for high volume and ongoing projects. Call for details. Standard turnaround is 4 business days and applies to pure cultures. Turnaround for Full Gene Sequencing is 5 business days and applies to pure cultures. *Requires 24 hour prior notification. Next Day and Same Day samples must be received by 8:00 am EST. Prices effective January 15, 2010 and are subject to change. Payment terms are Net 30 days.